mounting buffer dapi (Thermo Fisher)

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Thermo Fisher mounting buffer dapi
Mounting Buffer Dapi, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dapi+in+mounting+buffer/4++6+diamidino+2+phenylindole++dapi+/pm40615546-359-11-12
Average 90 stars, based on 1 article reviews

mounting buffer dapi - by Bioz Stars, 2026-10

90/100 stars

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Staining:

Article Title: Magnetotactic bacteria affiliated with diverse Pseudomonadota families biomineralize intracellular Ca-carbonate
Article Snippet: .. Slides were also stained with 4,6-diamidino-2-phenyl indole (DAPI) contained in the mounting solution (ProLong with DAPI, ThermoFisher Scientific) added to the slide. ..

Imaging:

Article Title: A flow cytometric approach to identifying the relative abundance and functional capacities of hemocyte subsets in the American cockroach, Periplaneta americana (L.)
Article Snippet: Sorted hemocytes were incubated with Phalloidin (F-actin, AAT Bioquest 23153) for 30 minutes at 30°C in a sterile microcentrifuge tube and washed 2x with insect media. .. The cell pellet was resuspended in ProLong TM Gold antifade mounting media with DAPI (Invitrogen P36935) and placed on a cover slip prior to imaging. .. Microscopy and imaging were carried out using a Zeiss Axio Observer ApoTome.2.

Incubation:

Article Title: CXCR4-LASP1-G9a-SNAIL axis drives NEPC transdifferentiation via induction of EMT and downregulation of REST.
Article Snippet: Fixed cells were then permeabilized with 0.5% Triton X-100 for 30 min. For the IF, fixed cells were incubated for 1 h in 3%BSA in PBS+0.1% Tween (PBS-T), and incubated overnight at 4◦C with primary antibodies, LASP1 (Santa Cruz, 374059) or Snail (Cell signaling, 3879). .. After washing with PBS-T, cells were incubated with secondary antibodies for 30 min, and mounted in mounting media with DAPI (ThermoFisher, P36931). .. Proximity Ligation Assay (PLA) was performed according to the manufacturers protocol with Duolink InSitu Fluoresence kit (Sigma-Aldrich, DUO92101), to analyze the interaction between LASP1 and Snail.

other:

Article Title: Generation and heterozygous repair of human iPSC lines from two individuals with the neurodevelopmental disorder, TRAPPC4 deficiency
Article Snippet: Fig. 6 Donor screening (OPTIONAL) HIV 1 + 2 Hepatitis B, Hepatitis C Not applicable Not applicable Blood group genotyping Not applicable Not applicableGenotype - additional histocompatibility info (OPTIONAL) HLA tissue typing Not applicable Not applicable Table 2: Reagents details Antibodies and stains used for immunocytochemistry/flow-cytometry Antibody Dilution Company Cat # and RRID Endoderm marker Mouse anti-SOX17 1:100 (#562205, BD BioScience) RRID: AB_10893402 Endoderm and Mesoderm marker Mouse anti-CXCR4 1:100 (#306514, BioLegend) RRID: AB_2089651 Mesoderm marker Goat anti-Brachyury 1:10 (#IC2085A, R&D Systems) RRID: AB_2891298 Ectoderm marker Mouse anti-Nestin 1:20 (#MA523650, Thermo Fisher) RRID: AB_2608686 Ectoderm marker Mouse anti-Pax-6 1:20 (#561552, BD BioScience) RRID: AB_10714781 Pluripotency marker; immunofluorescence Rabbit anti-Oct4 1:1000 (#ab19857, Abcam) RRID: AB_445175 Pluripotency marker; immunofluorescence Mouse anti-Nanog 1:2000 (#4893S, Cell Signaling Technology) RRID: AB_10548762 Pluripotency marker; FACS BV421 anti-human TRA-1–60 antibody 1:100 BD Biosciences Cat# 562,711 Pluripotency marker; FACS Alexa Fluor 647 antihuman TRA-1–81 antibody 1:100 BioLegend Cat# 330,706 Pluripotency marker; FACS PE/Cy7 anti-human SSEA-4 antibody 1:100 BioLegend Cat# 330,420 Pluripotency marker; FACS PE anti-human Oct3/4 antibody 1:100 Thermo Fisher Scientific Cat# 12– 5841-80 Western blot primary Rabbit anti-GAPDH 1:5000 (#G9545, Sigma-Aldrich) RRID: AB_796208 Western blot primary Mouse anti-TRAPPC4 1:500 (#H00051399-M01, Abnova) RRID: AB_425977 Secondary antibody HRP Sheep anti-mouse 1:5000 (#NA9310-1ml, Cytiva) RRID: AB_772193 Secondary antibody HRP Goat anti-rabbit 1:5000 (#7074S, Cell Signaling Technology) RRID: AB 2099233 Nuclear Stain DAPI In mounting media (#P36935, Thermo Fisher) Site-specific nuclease Nuclease information Nuclease type/version Cas9-Gem Delivery method Electroporation Neon Transfection System (Thermo Fisher) 1100 V, 30 ms, 1 pulse Selection/enrichment strategy Clonal selection Primers and Oligonucleotides used in this study Target Forward/Reverse primer (5′-3′) Clone screening by NGS TRAPPC4 variant TRAPPC4_scF: 5’-CAGCTGTCTCCTGAACAGG-3’ TRAPPC4_scR: 5’-CCCACCTGGAGATGCAAAC-3’ TRAPPC4 (RT-PCR) TRAPPC4 splicing F: 5’-CATCAATGGCATGGACGTGA-3’ R: 5’-ATGGATTCTTGAGGGCAAAGTCT-3’ Declaration of interests ☐ The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

Article Title: Encephalomyocarditis virus protein 2B* antagonises innate immune signalling by interacting with 14-3-3 protein family members
Article Snippet: DAPI was included in the mounting media (Prolong gold antifade with DAPI, ThermoFisher Scientific).

Article Title: Precision Medicine: IL-1RA and Pancreatic Cancer Organoids.
Article Snippet: Organoid gels were mounted in Fluoromount-G mounting solution with DAPI (Cat#00-4959-52; ThermoFisher Scientific).

Article Title: Intestinal lymphatic vasculature is functionally adapted to different drainage regions and is altered by helminth infection.
Article Snippet: Tissues were washed a final three times for 30 min each at RT to remove excess unbound secondary antibody and mounted in DAPI Fluoromount-G clear mounting media (Thermo Fisher Scientific).

Article Title: Intestinal lymphatic vasculature is functionally adapted to different drainage regions and is altered by helminth infection.
Article Snippet: Slides were mounted in DAPI Fluoromount-G clear mounting media (Thermo Fisher Scientific) and covered with a glass coverslip for imaging.

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